Selective expansion of mitochondrial nucleoside triphosphate pools in antimetabolite-treated HeLa cells.
نویسندگان
چکیده
منابع مشابه
Origins of mitochondrial thymidine triphosphate: dynamic relations to cytosolic pools.
Nuclear and mitochondrial (mt) DNA replication occur within two physically separated compartments and on different time scales. Both require a balanced supply of dNTPs. During S phase, dNTPs for nuclear DNA are synthesized de novo from ribonucleotides and by salvage of thymidine in the cytosol. Mitochondria contain specific kinases for salvage of deoxyribonucleosides that may provide a compartm...
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The influence of mutations in the mitochondrial DNA (mtDNA) on the bioenergetic metabolism of the cell is still poorly understood. Many of the mutations in the mtDNA affect the expression of the mitochondrial genome. Investigations on cells from patients are not easy, especially as the mitochondrial DNA is heteroplasmic and this state is changed in culture. Moreover, the nuclear background and ...
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Amino acid starvation of "stringent RNA control" (RC(str)) strains of Escherichia coli (E. coli) results in the cessation of net protein and net RNA synthesis, whereas "relaxed RNA control" (RC(rel)) strains continue net RNA synthesis under the same conditions of amino acid starvation. This report tests further the hypothesis that net RNA synthesis is markedly reduced during amino acid starvati...
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The two nucleic acid-dependent nucleoside triphosphate phosphohydrolases, previously purified from vaccinia virus cores, were shown to be immunologically distinct enzymes. Antiserum prepared against purified phosphohydrolase I and antiserum prepared against purified phosphohydrolase II only neutralized the activity of that enzyme used as antigen. Both enzymes were induced in HeLa cells after va...
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The mode of mitochondrial formation has been so far directly investigated only in Neurospora crassa. By using a choline-requMng mutant of this organism, Luck (1963, 1965) has obtained evidence which strongly suggests that mitochondria are formed by growth and division of preexisting mitochondria. In Tetrahymena pyriformis (Parsons and Rustad, 1968), the distribution of long-term [aH]thymidine-l...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34068-7